IgM as the first responder: how does its size and

From what I’ve seen messing around with similar assays, IgM can be a little tricky early on, mostly because it behaves differently than smaller antibodies. I remember double-checking basics like the lgm full form when I first hit this wall, just to reset my own understanding. The J-chain and overall structure seem to influence how things bind and signal, at least anecdotally. In my case, results weren’t wrong, just more variable than I expected, which made interpretation feel less straightforward.